And PDGF-BB concentration released from P-PRG activated with CG at 6 h. The findings of our study suggest that CG produces a complete release of GFTable 5 Hematological results for gender and their respective blood componentsMale Variable Whole blood RBC (106/L) WBC (103/L) LY ( ) MO ( )* EOS ( )* GRA ( ) PLT (103/L) MPV (fL) PDW ( ) 6.5 (0.7)a 8.0 (1.5)a 35.9 (8.8)aFemale (n=10) PPP 0.0 (0.0) 0.0 (0.0) ----99.8 (23.3) 3.4 (0.4) 18.5 (0.7) Whole blood 6.2 (0.9)a 8.8 (1.7)b,c 40.5 (7.2) 1.8 (1.5)a b(n=30) P-PRP 0.1 (0.1)b 4.0 (1.8)b 58.2 (9.9)bP-PRP 0.1 (0.1)b 5.2 (3.1)b 60.6 (8.6)b 0.0 (1.6)dPPP 0.0 (0.0) 0.0 (0.0) ----120.0 (26.8) 3.2 (0.2) 18.2 (0.8)1.8 (0.8) 3.1 (3.8) 59.6 (9.4)a0.0 (0.9) 0.0 (4.0) 39.2 (10.4)b5.1 (5.2)b 52.7 (9.0)b,c0.0 (3.8)d 35.8 (11.7)b159.7 (22.7)a 3.4 (0.4)c 16.9 (0.7)b295.7 (44.2)b 3.6 (1.1)b 17.1 (0.5)b189.1 (22.7)a 3.4 (0.3) 16.6 (0.8)b329.9 (32.7)b 3.7 (0.2)d 17.0 (0.5)bData are presented as mean ( .d.). The initials like in Table 1. Letters denote SD for each independent sex in each 2-Bromo-1,3-difluoro-4-nitrobenzene blood component. a: SD (P<0.001) from P-PRP and, PPP. b: SD (P<0.001) from PPP. c: SD (P<0.05) from P-PRP. d: SD (P<0.05) from PPP. : variable SD (P<0.001) between males and females. : variable SD (P<0.05) between males and females. *Data are presented as median (IR).Giraldo et al. BMC Veterinary Research 2013, 9:29 http://www.biomedcentral.com/1746-6148/9/Page 6 ofTable 6 Results for total protein, transforming growth factor beta 1 and platelet derived growth PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20618955 factor isoform BB for gender and their respective blood componentsVariable Males (n=30) TP (mg/mL)* TGF-1 (pg/mL) TGF-1 (pg/mg of TP) PDGF-BB (pg/mL) PDGF-BB (pg/mg of TP) Females (n=10) TP (mg/mL)* TGF-1 (pg/mL) TGF-1 (pg/mg of TP) PDGF-BB (pg/mL) PDGF-BB (pg/mg of TP) 45.0 (5.1) 1185.6 (508.9)f 26.7 (12.5)f 333.6 (477.8)gBlood component Plasma 47.1 (4.6) 1019.0 (467.8)b,cP-PRP+NID 4-Bromopicolinaldehyde 49.4 (6.6)a 3325.8 (1108.1)d 69.4 (28.2)d 1060.5 (279.0)dP-PRG 46.2 (4.0) 2536.6 (582.3)d 54.9 (14.8)d 978.1 (284.7)d 21.1 (6.9)d 43.5 (6.7) 2892.8 (1749.0) 64.9 (37.1) 1589.1 (363.7)h 36.8 (10.3)hPPP+NID 46.7 (3.4) 1674.8 (533.3) 35.6 (11.1) 428.7 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/12711626 (135.2)?9.2 (3.6)?PPG 46.8 (5.6) 1512.6 (549.7) 32.5 (13.4) 418.2 (103.0) 8.8 (2.5)21.9 (11.6)b,c 69.9 (50.7)e1.5 (1.1)e22.0 (7.2)d 49.5 (7.8) 3608.0 (1906.2)c 71.2 (34.8)c 1535.5 (444.6)h 30.6 (8.0)44.3 (5.1) 1704.8 (504.4) 37.9 (11.3) 864.0 (463.0) 19.4 (10.8)42.8 (4.7) 1410.0 (197.4) 32.8 (4.9) 933.7 (319.9) 21.7 (7.7)7.7 (11.2)iData are presented as mean ( .d.). The initials like in Table 2. Letters denote SD for each independent sex and their respective blood components. a: SD (P<0.05) from P-PRG. b: SD (P<0.001) from P-PRP+NID, P-PRG and, PPP+NID. c: SD (P<0.05) from PPG. d: SD (P<0.001) from PPP+NID and, PPG. e: SD (P<0.001) from P-PRP+NID, P-PRG, PPP+NID and, PPG. f: SD (P<0.05) from P-PRP+NID. g: SD (P<0.001) from P-PRP+NID and, P-PRG. h: SD (P<0.05) from PPP+NID and, PPG. i: SD (P<0.05) from P-PRP+NID, P-PRG, PPP+NID and, PPG. : variable SD (P<0.05) between males and females. : variable SD (P<0.001) between males and females. ? variable SD (P<0.003) between males and females. *Data are presented as median (IR).from P-PRG in the first 6 h post-activation. In contrast, COL1 produced a very weak GF release from PRP [10]. Our study demonstrated that intrinsic factors such as, breed, gender and age influence the cellular composition of P-PRP and GF content in P-PRG. These findings are novel and should be considered in the clinical use of these.